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- 1). Compute intensity ratio (Io/I). Divide original fluorescence intensity (Io) to fluorescence intensity measured after a specified time (I) with the aid of a calculator to obtain a dimensionless value.
- 2). Evaluate the "logarithm to base e of intensity ratio." Evaluate a value for "Ln(Io/I)" with the aid of a calculator to obtain a single dimensionless value.
- 3). Determine the reciprocal of "logarithm to base e of intensity ratio." Divide one (1) by the value for "Ln(Io/I)" with the aid of a calculator to obtain a single dimensionless value.
- 4). Calculate theoretical fluorescence lifetime. Multiply measured time (t) in seconds by the reciprocal of "logarithm to base e of intensity ratio" with the aid of a calculator to obtain a theoretical fluorescence lifetime in seconds.
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