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- The bacterial stain, Ziehl-Neelsen stain, stains acid-fast bacteria such as mycobacteria. The stain includes carbol fuchsin, a mixture of phenol and fuchsin, acid alcohol and methylene blue. The bacteria will stain red.
- The mixture of picric acid and acid fuchsin, or the Van Gieson's stain, is useful to examine collagen. The collagen will stain a pink color, while the nucleus stains brown and tissue such as muscle, red blood cells and fibrin will stain yellow.
- The combination of basic fuchsin and methylene blue is known as Lee's stain, and is a general purpose stain. The cell's nuclei will stain blue, cartilage will be a blue/purple and the cytoplasm and mitochondria will stain red.
- Take care while using all stains and dyes. Wear correct protective equipment such as gloves and goggle. Avoid chemicals such as phenol and picric acid where possible and use alternatives.
Staining Bacteria
Staining Collagen
General Purpose Stain
Caution
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